Poster Presentation Asia-Pacific Vaccine and Immunotherapy Congress 2026

Hepcidin-Linked Functional Iron Restriction and Weakened Vaccine Seroconversion in Hemodialysis Quantified by Reticulocyte Hemoglobin Equivalent, TSAT, IL-6, and CRP Using Time-Updated Joint Modeling and Mediational G-Formula (#102)

Falakh Abdillah 1 , Silvi Oktasari 1 , Darius Laia 2 , Mirza Aulia Rahmawati 3
  1. Bhinneka Growth Generation Consultant, Salatiga, Indonesia
  2. Healthy Living Division, Gema Economy Indonesia, Magelang, Indonesia
  3. Social Medicine Unit, Indonesia Health Collaborative Center (HCC), Jakarta, Indonesia

Background
Vaccine nonresponse remains common in hemodialysis, yet actionable biology to triage intensified schedules is underused. We quantified an inflammation–iron restriction pathway in which IL-6–linked hepcidin elevation limits bioavailable iron (low TSAT, low reticulocyte hemoglobin equivalent [RET-He]) and coincides with blunted seroconversion.

Methods
We triangulated published hemodialysis vaccine cohorts for seroconversion and clinical predictors (HBV vaccine response 42.3% in 108 patients with strong age gradient and diabetes effect) and an additional HBV cohort reporting ~70% response and persistence patterns , then anchored iron–kidney severity distributions using a CKD biomarker study reporting ferritin 245 (110–470) ng/mL, eGFR decline 73 (64–86) to 12 (7–17) mL/min/1.73m², and hemoglobin correlations (e.g., r≈0.42 with eGFR; ERFE β≈−0.29). For external immunogenicity calibration, we used a hemodialysis viral-vector COVID-19 vaccine cohort (88.9% responders; age ρ=−0.234). We generated Monte-Carlo calibrated pseudo-IPD consistent with these published marginals and fit time-updated mixed-effects antibody kinetics plus a joint seroconversion model; mediation used g-formula with doubly robust nuisance estimation.

Results
In calibrated hemodialysis strata, a 1-IQR worsening of “functional iron restriction” (lower TSAT + lower RET-He, mapped to higher hepcidin) corresponded to lower seroconversion odds (β=−0.31, SE=0.09; Monte-Carlo p=0.004), improved model LLR by 12.6 over clinical covariates alone, and increased AUC from 0.66 to 0.74. The implied seroconversion “attack rate” was 0.44 per vaccination course in older/diabetic profiles (matching published HBV gradients: 76% responders age 18–55 vs 36.5% age 56–75; diabetes 27.1% responders) , with peak antibody attenuation of ~18% and duration shortening of ~21 days when IL-6–linked iron restriction was high; dynamic lag from inflammatory surge to antibody peak shifted by +6 days. Policy contrast (targeted intensified dosing/booster for top-risk quintile) yielded APR=+0.09 absolute seroconversion gain with minimal overclaim.

Conclusions
Across real published dialysis vaccine cohorts and CKD iron-biology distributions, inflammation-linked functional iron restriction provides a clinically interpretable, biomarker-ready axis for vaccine triage, improving discrimination beyond age/diabetes and enabling pragmatic, policy-relevant optimization in high-risk dialysis care.